Figure 6.

Proliferating cell nuclear antigen (PCNA) and p53 expression in the mouse model of inflammatory bowel disease. (a–c) Double immunostaining for 8‐nitroguanine and PCNA. (a,d) 8‐Nitroguanine localizes widely in the mucosa, (b) intense PCNA is expressed in the nuclei of many glandular epithelial cells and infiltrated cells in lamina propria, (c) PCNA is expressed partially in the nuclei of 8‐nitroguanine‐immunoreactive epithelial cells, (d–f) double immunostaining for 8‐nitroguanine and p53, (e) significant p53 expression is observed in the nuclei of regenerated epithelial cells (arrowhead) and some infiltrated cells (small arrow). p53 is also induced in the cytoplasm of many epithelial cells in lower portions of the gland (large arrow). (f) p53 is expressed in most of 8‐nitroguanine‐immunoreactive epithelial cells. Scale bar: 50 µm.