Figure 3.

Morphogenesis and differentiation in vitro of GIF‐11 cells cultured with (a–c) collagen gels, (d–f) gastric mesenchymes, and (g–i) Matrigel, for (a,d,g) 1, (b,e,h) 2, and (c,f,i) 3 weeks. Sections are stained with periodic acid–Schiff (PAS) and hematoxylin. (f) Closed arrows show surface mucous cells characterized by large PAS‐positive materials and elongated nuclei. Some fibroblasts die during long‐term culture, and open arrows in (f) show the nuclei of dying cells. No leukocytes can be found in the mesenchyme in organ culture. Scale bars = 100 µm.