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. 2008 Mar 3;99(4):671–676. doi: 10.1111/j.1349-7006.2008.00732.x

Figure 4.

Figure 4

Differentiation of mucus‐containing cells in an adult mouse (a,c,e) stomach and (b,d,f) small intestine, and in GIF‐11 cells cultured with Matrigel for (g–i) 4 and (j–l) 6 weeks. Periodic acid–Schiff (PAS) and hematoxylin staining shows that (g,j) some GIF‐11 cells contain large PAS‐positive mucus droplets (arrows), (b) found specifically in goblet cells in the intestine (arrows). Alcian blue (AB)‐neutral red and high iron diamine (HID)‐AB staining shows that (h,k) the mucus droplets in GIF‐11 cells are AB‐positive (arrows) and (i,l) HID‐positive (arrows), (d,f) as is the case for mucus in goblet cells, (c,e) whereas mucus in gastric epithelial cells is negative for both stains. Scale bars = 50 µm.