Figure 5.

Reverse transcription–polymerase chain reaction analysis on the differentiation of GIF‐11 cells combined with collagen gel (middle panel) or Matrigel (right panel) for 1 day (1 d), 2 weeks (2 w), 4 weeks (4 w), and 6 weeks (6 w). Mucin1 (Muc1), Sox2, intrinsic factor (IF), Mucin2 (Muc2), Cdx2, and sucrase isomaltase (SI) are used as tissue‐specific markers for differentiation, as Muc1, Sox2, and IF are expressed explicitly by gastric (stomach) epithelial cells, and Muc2, Cdx2, and SI are expressed specifically by intestinal (duodenum) epithelial cells (left panel). Gapdh, glyceraldehyde 3‐phosphate dehydrogenase.