Table 1.
Primers used for PCR and sequencing for screening of mutations
| Gene | Sense | Antisense | Sequencing |
|---|---|---|---|
| PIK3CA | |||
| Exon 9 | 5′‐GATTGGTTCTTTCCTGTCTCTG‐3′ | 5′‐CCACAAATATCAATTTACAACCATTG‐3′ | 5′‐TTGCTTTTTCTGTAAATCATCTGTG‐3′ |
| Exon 20 | 5′‐TGGGGTAAAGGGAATCAAAAG‐3′ | 5′‐CCTATGCAATCGGTCTTTGC‐3′ | 5′‐TGACATTTGAGCAAAGACCTG‐3′ |
| EGFR | |||
| Exon 18 | 5′‐TCAGAGCCTGTGTTTCTACCAA‐3′ | 5′‐TGGTCTCACAGGACCACTGATT‐3′ | 5′‐TCCAAATGAGCTGGCAAGTG‐3′ |
| Exon 19 | 5′‐AAATAATCAGTGTGATTCGTGGAG‐3′ | 5′‐GAGGCCAGTGCTGTCTCTAAGG‐3′ | 5′‐GTGCATCGCTGGTAACATCC‐3′ |
| Exon 21 | 5′‐GCAGCGGGTTACATCTTCTTTC‐3′ | 5′‐CAGCTCTGGCTCACACTACCAG‐3′ | 5′‐GCTCAGAGCCTGGCATGAA‐3′ |
| PTEN | |||
| Exon 1 | 5′‐GCAGCTTCTGCCACTTCTCT‐3′ | 5′‐CATCCGTCTACTCCCACGTT‐3′ | |
| Exon 2 | 5′‐CTCCAGCTATAGTGGGGAAAA‐3′ | 5′‐CTGTATCCCCCTGAAGTCCA‐3′ | |
| Exon 3 | 5′‐TGGTGGCTTTTTGTTTGTTT‐3′ | 5′‐CATGAATCTGTGCCAACAATG‐3′ | |
| Exon 4 | 5′‐AAAGATTCAGGCAATGTTTGTT‐3′ | 5′‐TCTCACTCGATAATCTGGATGAC‐3′ | |
| Exon 5 | 5′‐GGAATCCAGTGTTTCTTTTAAATACC‐3′ | 5′‐TCCAGGAAGAGGAAAGGAAAA‐3′ | |
| Exon 6 | 5′‐ATGGCTACGACCCAGTTACC‐3′ | 5′‐TTGGCTTCTTTAGCCCAATG‐3′ | |
| Exon 7 | 5′‐TGCTTGAGATCAAGATTGCAG‐3′ | 5′‐GCCATAAGGCCTTTTCCTTC‐3′ | |
| Exon 8–1 | 5′‐GTGCAGATAATGACAAGGAATA‐3′ | 5′‐ACACATCACATACATACAAGTC‐3′ | |
| Exon 8–2 | 5′‐TTAAATATGTCATTTCATTTCTTTTTC‐3′ | 5′‐CTTTGTCTTTATTTGCTTTGT‐3′ | |
| Exon 9 | 5′‐TGTTCATCTGCAAAATGGAAT‐3′ | 5′‐CAAGTGTCAAAACCCTGTGG‐3′ | |