Figure 1.

Establishment of an RH7777 rat hepatoma cell line expressing green fluorescent protein (GFP)‐human l‐type amino‐acid transporter (LAT) 1. (a) RH7777 cells were transfected with GFP‐human LAT1 cDNA using Lipofectamine 2000. The transfected cells were cultured in Dulbecco's modified Eagle's medium containing 7% fetal bovine serum and 400 µg/mL G418 for 2 weeks (left), and cells strongly expressing GFP‐human LAT1 proteins were clone sorted and expanded (right). (b) Phase‐contrast micrographs (left) and micrographs of ultraviolet‐excited green‐colored GFP‐LAT1 proteins (right) of clone‐sorted cells.