Figure 3.

Expression of l‐type amino‐acid transporter (LAT) 1 protein and mRNA in various human cell lines. (a) Unfixed HeLa cells, and (b) unfixed and paraformaldehyde (PFA)‐fixed human cell lines were stained with SOL22, followed by fluorescein isothiocyanate (FITC)‐conjugated anti‐rat IgG Fcγ, and analyzed by flow cytometry. Dotted lines show control cells without the first monoclonal antibody (mAb), while solid lines show cells stained with mAb (a). A statistical analysis was carried out using CellQuest software, and mean fluorescence intensity (MFI) was subtracted from values without the first antibody (b). (c) Real time quantitative reverse transcription–polymerase chain reaction was carried out. The expression of LAT1 mRNA was normalized to the expression of glyceraldehyde‐3‐phosphate dehydrogenase mRNA in each cell line.