Figure 2.

Bioluminescence imaging of p53 induction in HCT116‐p53RE‐Luc tumor‐bearing BALB/c nude mice. Mice bearing a p53^+/+ tumor on the left flank and a p53^−/– tumor on the right flank were imaged before and 24 h after receiving an intraperitoneal injection of doxorubicin (1.5 mg/kg). (a) Gray‐scale image of a representative mouse overlayed with a pseudo‐color image of bioluminescence. All window and level settings depict a range between 8000 and 250 000 photons/s and are consistent for all images. (b) quantification of bioluminescence on tumor regions of interest (ROI) before and after treatment with doxorubicin. Columns, averaged bioluminescence (n = 5 mice) expressed as total photons flux in the ROI; bars, standard error of the mean. *P = 0.0103; NS: non‐significant (P = 0.4207).