Figure 5.

Ovalbumin (OVA)‐major histocompatibility complex (MHC) tetramer⁺ CD8⁺ cells were efficiently mobilized into draining lymph nodes (DLN) from mice treated with anti‐OX40 monoclonal antibody (mAb) in combination with radiotherapy. The Lewis lung carcinoma‐OVA‐bearing mice were treated with the protocols as described in Materials and Methods. DLN obtained from treated mice were labeled with phycoerythrin‐conjugated OVA‐MHC tetramer, followed by staining with fluorescein isothiocyanate‐conjugated anti‐CD8 mAb. (a) Representative data from flow cytometric analysis. The numbers in each graph indicate the percentage of OVA‐MHC tetramer⁺ cells in total cells from DLN. (b) Flow cytometric analysis pooled from four separate analyses. Data are presented as the mean ± SE. *P = 0.005. RT, radiotherapy.