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. 2006 Mar 30;97(4):259–270. doi: 10.1111/j.1349-7006.2006.00169.x

Figure 2.

Figure 2

Induction of morphological changes in various types of cancer cells by constitutive expression of the WT1 17AA(–)/KTS(–) isoform. (a) Green fluorescent protein (GFP)‐tagged WT1 17AA(–)/KTS(–) isoform was expressed transiently in various types of cancer cells such as ZR‐75, HT‐1080, MKN28, SKBr3 and TE10. Cells were analyzed morphologically using confocal microscopy 48–72 h after transfection. Upper panel, transparent images; lower panel, fluorescence images. Arrows indicate the cells expressing GFP‐tagged WT1 17AA(–)/KTS(–) protein. Scale bars = 10 µm. (b) Means of relative areas of more than nine individual cells. Areas were calculated using NIH Image software.