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. 2006 Mar 30;97(4):259–270. doi: 10.1111/j.1349-7006.2006.00169.x

Figure 7.

Figure 7

Suppression of gelsolin expression reduces cell migration but does not effect morphology or cell–substratum adhesion. WT1 17AA(–)/KTS(–) isoform‐transduced TYK cells were transduced with a gelsolin‐specific small interfering RNA (siRNA) vector (TW/GSNsiRNA) or control vector (TW/siMock). (a) Expression levels of gelsolin protein determined by western blot analysis. Three different cell clones were examined. (b) Cells were stained with MayGrünwald–Giemsa. Scale bars = 10 µm. (c) Areas of TW/Mock cell clone and TW/GSNsiRNA were calculated using NIH Image software. (d) Cell–substratum adhesion determined by detachment assay. (e) Collective cell motility evaluated by wound healing assay. (f) Expression levels of α‐actinin 1 and cofilin determined by western blot analysis. (c–e) (▪), Cell clones transduced with siRNA vector specific for gelsolin; (□), mock siRNA vector‐transduced cell clones.