Table 1.
Polymerase chain reaction primers for amplification of actin binding proteins
| Gene | Sense primer sequence | Antisense primer sequence |
|---|---|---|
| Gelsolin † | CTT TCC AGC CAT ATC GCC AAC | TTC TCT GCC TCG CTG GCT C |
| Profilin † | GGC CAG AAA TGT TCG GTG | ACG GGA GGG ATA TGG GTA |
| p125fak † | GAC TCA CCT GGG TAC TGG TAT G | ATC GCT CTT CAC CTG TTG ATA G |
| Paxillin † | CAG TCG CCA AAG GAG TCT G | GTA GTC CTT GCG ACA GTA GGC |
| α‐Actinin 1 † | GGA GCC GAA GAA ATC GTG | CTG CTC GTT CTC CTG GTT G |
| VASP † | GGA AAG TCA GCA AGC AGG | TGT GCG GAA AGG AGA AGC |
| Talin | TGG CTA CCT GGA ACT GCT GGA C | TCC AGC TCT CGT TCC TTC CGA AG |
| Cofilin | AGT CTT CAA CGC CAG AGG AGG TG | GTG CAG CGG TCC TTG ACC TCC T |
| Ezrin | AGG AGT TGA TGC TGC GGC TGC A | GTG GAT GAT GTC ATT GTG GGT CC |
| Radixin | ACC ACC AGT CAT TCC TCC AAC AG | GGC AAG GTG GGA TGC ATT CCA TC |
| Moesin | TGG TGC CTT CAA GAC CTT CAC C | GTC ACC TGA GAG GGT TGA GTA AAC |
| GAPDH | GCC AAA AGG GTC ATC ATC TC | GTA GAG GCA GGG ATG ATG TTC |
†
Primers reported previously.( 30 ) mRNA of actin binding proteins was amplified by reverse transcription–polymerase chain reaction (20 cycles, annealing temperature 60°C) using the pairs of primers shown above. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; VASP, vasodilator‐stimulated phosphoprotein.