Figure 2.

hPER3 gene expression and methylation‐specific PCR. (a) hPER3 gene expression levels of three representative CML cases as measured by real‐time quantitative RT‐PCR. In the same CML patient, hPER3 expression level was higher at chronic phase and was lower at blastic crisis. (b) Methylation‐specific PCR of PER3. M and U indicate methylation‐specific PCR using PER3‐M and PER3‐U primer sets, respectively. CP and BC indicate chronic phase and blastic crisis of CML, respectively. Marker represents the 100‐bp ladder DNA marker. Patients 1, 2, and 3 correspond to the same patients in (a).