Figure 5.

Demethylation studies using K562 cells treated with 5‐aza‐2′‐deoxycytidine and/or Trichostatin A. 10⁶ cells were treated with 1 µM 5‐aza‐2′‐deoxycytidine or/and 100 nM Trichostatin A for 1, 2, 3, 4, and 5 days respectively then harvested for hPER3 expression and methylation‐specific PCR analysis. (a) hPER3 gene expression levels in K562 cells were increased after 5‐aza‐2′‐deoxycytidine, Trichostatin A, and combined treatments for two days and persisted for 5 days as determined by real‐time quantitative RT‐PCR. Data are presented as folds increased compared to untreated K562 cells. Each data point was calculated from 3 duplicate studies. (b) Methylation‐specific PCR of PER3‐M and PER3‐U of K562 cells after 5‐aza‐2′‐deoxycytidine treatment. PER3‐M PCR products showed a time‐dependent decrease of intensities and PER3‐U PCR products showed a slight increase of intensities. Marker represents the 100‐bp ladder DNA marker.