Table 1.

Oligonucleotide primers and probes for real‐time quantitative reverse transcription–polymerase chain reaction analysis of the nine circadian genes

Gene	GenBank accession no.	Amplicon size (bp)	Direction	Sequence	Location
PER1	AF022991	102	F	5′‐CAT CAC GTC TGA GTA CAC ACT TCA GA‐3′	808–833
			P	5′‐CGA ATC GTC TAC ATT TC‐3′	875–891
			R	5′‐AGG ACG GCT GCC TGC TC‐3′	893–909
PER2	NM_003894	98	F	5′‐TCC AGA TAC CTT TAG CCT GAT GA‐3′	353–375
			P	5′‐CCA TCT ACA AGT GGC TGC‐3′	396–413
			R	5′‐TTT GTG TGT GTC CAC TTT CGA‐3′	429–449
PER3	NM_016831	94	F	5′‐ACA AAC AGA ACC ACA AGG CA‐3′	1893–1912
			P	5′‐CTT ACA AGC TGG TTT GC‐3′	1927–1943
			R	5′‐CGT CCA TTT GTT GGC ATT T‐3′	1986–1968
CRY1	NM_004075	91	F	5′‐AGA ACA GAT CCC AAT GGA GAC T‐3′	1847–1868
			P	5′‐TAT CAG GCG TTA TTT GCC TGT‐3′	1830–1830
			R	5′‐GTG CAT TCC AGG GAT CAT AGA‐3′	1917–1937
CRY2	XM_051030	86	F	5′‐CCT CCT CAG TCG GGA TCA A‐3′	231–249
			P	5′‐CGA TGG AGG TTC CTA C‐3′	251–266
			R	5′‐GCG GGA GTT CAG TTT CCT TA‐3′	297–316
CLOCK	AF011568	107	F	5′‐CAA CGC ACA CAT AGG CCA T‐3′	1069–1087
			P	5′‐CAG GTT AGC TAC ACC TCA G‐3′	1122–1140
			R	5′‐TTG GGT TCT TCA ACA GTG CA‐3′	1156–1175
BMAL1	D89722	97	F	5′‐CAT GCA ACG CAA TGT CCA G‐3′	318–336
			P	5′‐CTG TGC TAA GGA TGG CT‐3′	354–370
			R	5′‐GTG TAT GGA TTG GTG GCA CCT‐3′	394–414
CKI∈	NM_152221	110	F	5′‐AGG TCC TCT GCA AAG GCT ATC C‐3′	945–966
			P	5′‐CGA ATT CTC AAC ATA CCT CAA‐3′	970–990
			R	5′‐GCG GAA GAG CTG ACG TAG GTA A‐3′	1033–1054
TIM	NM_003920	97	F	5′‐CAG CAC CAC CAG GAC AAG C‐3′	353–371
			P	5′‐CTG ATG GTG AAC TTG ACA C‐3′	395–413
			R	5′‐GCA GAT TGC CAA AAC AGA GCA‐3′	423–443

F, forward; P, probe; R, reverse.