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Recombinant hsc70 and fusion proteins used in this study. Cytotoxic T lymphocyte epitopes OVA257‐264 and TRP2180‐188 were fused to the C‐terminus of hsc70. (d–f) In some constructs, the helper T cell epitope OVA265‐280 was fused to the N‐terminus of hsc70. Recombinant proteins also contained an N‐terminal 6 × His‐tag. They were expressed in E. coli, M15, and purified with Ni‐NTA columns.
