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. 2008 Mar 12;99(5):1008–1015. doi: 10.1111/j.1349-7006.2008.00788.x

Figure 3.

Figure 3

Comparison of the efficiency of cytotoxic T lymphocyte (CTL) generation by vaccination with hsc70 fusion proteins or synthetic peptides. C57BL/6 mice were immunized intraperitoneally with the indicated doses of synthetic peptides plus incomplete Freund's adjuvant (IFA) or with 10 µg (or the indicated volume) hsc70 fusion proteins, twice in a 1‐week interval. Two weeks after the second immunization, the spleen cells were stimulated in vitro with 10−6 M OVA257‐264 or TRP2180‐188 for 5 days. CTL activity against E.G7, EL4, and EL4 pulsed with OVA257‐264 was examined using a standard 51Cr‐release assay.