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Analysis of cell cycle distribution. Cells treated with gefitinib (2.0 µM) for 72 h were pulse‐labeled with 5‐bromodeoxyuridine (BrdU) and stained with anti‐BrdU antibody and PI (left panel). Numbers in lower middle, upper, lower right, and left regions indicate the percentages of cells in the G1, S, G2/M phases, and with sub‐G1 DNA content, respectively. In the right panel, these data are shown as a bar graph.
