Figure 1.

Sensitivity for detection of the E746_A750del and L858R mutations with the epidermal growth factor receptor (EGFR) Scorpion Kit. (A) Standard E746_A750del DNA was used at various volumes: 10 000 pg (10⁴), 1000 pg (10³), 100 pg (10²) and 10 pg (10¹). Standard wild‐type DNA (Wild) and distilled water (DW), as negative controls, were used in the same experiment. (B) Standard E746_A750del DNA (10–10 000 pg) was mixed with 10 000 pg of standard wild‐type DNA at ratios of 1 : 1 (10), 1 : 10 (10⁻¹), 1 : 100 (10⁻²) and 1 : 1000 (10⁻³). (C) Standard curves were obtained by plotting the threshold cycle (Ct) of each curve (shown in Fig. 1A,B) against the log of the standard DNA volume. Detection of E746_A750del and L858R in plasmid DNA derived from lung cancer cell lines. (D) PC‐9 with E746_A750del DNA and A431 with wild‐type DNA. (E) 11‐18 with L858R DNA and A431.