Figure 2.
Results of direct sequencing and the Scorpion Amplified Refractory Mutation System (ARMS) method in patient no. 10 (A,B) and patient no. 21 (C,D). (A) The wave figure represents wild‐type epidermal growth factor receptor (EGFR). (B) Two ascending curves, indicating that wild type and deletion mutation in exon 19 were detected. (C) The two waves start to overlap at the starting points of the arrows. These features mean that the nucleotide sequence of the EGFR gene in this patient has a heterozygous deletion. The deletion removed amino acids 746–750 (E746_A750del). (D) Two ascending curves, indicating that wild type and deletion mutation in exon 19 were detected.