Table 2.
Flow cytometry to compare T cell subpopulations. The top panel used alive cells as the parental gate. The bottom panel used CD45+ alive cells (total immune cells) as the parental gate. (Abbreviations: SSC-A: Side SCcatter parameter A; GRZB: granzyme B; PERF: perforin; Tregs: T regulatory cells, GITR: Glucocorticoid-Induced TNFR-Related protein; Tconv: T conventional cells, defined as CD4+FOXP3-CD25low; CD8+ naïve T cells: defined as CD8+CCR7+CD45RA+; Avg: Average; Diff: difference)
| Population | Avg, EGFR | Avg, WT | Diff. | p Value |
|---|---|---|---|---|
| CD45+ SSC-A subset | 32.47 | 32.59 | −0.12 | 0.991 |
| CD3+ | 20.05 | 17.39 | 2.66 | 0.730 |
| CD4+ | 12.79 | 9.42 | 3.37 | 0.457 |
| CD8+ | 6.09 | 6.67 | −0.58 | 0.840 |
| Population | Avg, EGFR | Avg, WT | Diff. | p Value |
| CD8+ | 25.58 | 33.53 | −7.94 | 0.079 |
| CD8+ PD1+ | 7.80 | 20.12 | −12.32 | 0.000 |
| CD8+ TIM3+ | 0.81 | 2.87 | −2.07 | 0.001 |
| CD8+ Ki67+ | 2.41 | 4.53 | −2.12 | 0.028 |
| CD8+ GRZB+ PD1+ | 0.35 | 1.25 | −0.90 | 0.016 |
| CD8+ GRZB+ PERF1+ | 0.62 | 2.18 | −1.56 | 0.026 |
| CD8+ CD103+ | 7.20 | 13.22 | −6.02 | 0.045 |
| CD8+ CD103- | 12.82 | 10.31 | 2.51 | 0.415 |
| CD8+ Naïve | 0.06 | 0.25 | −0.19 | 0.039 |
| CD4+ | 64.03 | 53.70 | 10.33 | 0.046 |
| CD4+ Tregs | 1.32 | 1.25 | 0.06 | 0.911 |
| CD4+ Tregs GITR+ | 1.66 | 0.40 | 1.26 | 0.001 |
| CD4+ Tconv | 39.56 | 30.66 | 8.90 | 0.072 |