Table 6.
Number of frameshift mutations detected in cell-free DNA from high microsatellite instability patients
| Sample ID | Variants, No. | Targets, No. | Cell-free DNA yield, ng |
|---|---|---|---|
| Heidelberg | |||
| P1a | 4 | 4 | 6.10 |
| P2a | 2 | 2 | 3.67 |
| P3a | 1 | 1 | 9.16 |
| P4a | 23 | 21 | 12.07 |
| P5 | 105 | 81 | 10.84 |
| P6 | 9 | 9 | 5.91 |
| P7a | 3 | 3 | 4.90 |
| P8 | 2 | 2 | 6.16 |
| P9a | 1 | 1 | 4.76 |
| P10a | 2 | 2 | 4.96 |
| P11 | 3 | 3 | 2.22 |
| P12 | 7 | 6 | 1.74 |
| P13 | 7 | 7 | 2.29 |
| P14 | 8 | 8 | 1 |
| P15 | 47 | 45 | 1.65 |
| P16 | 104 | 79 | 10.25 |
| P17a | 3 | 3 | < 1 |
| Weill Cornell | |||
| P20-1b | 2 | 2 | 11.33 |
| P20-2b | 11 | 10 | 10.63 |
| P21 | 4 | 4 | 64.9 |
| P22 | 11 | 10 | 177.1 |
| P23 | 18 | 17 | 56.1 |
| Tissue For Research Ltd | |||
| P50 | 72 | 61 | 12.43 |
| P51 | 61 | 56 | 4.71 |
| P52 | 6 | 6 | 7.74 |
| P53a | 14 | 14 | 2.51 |
| P54a | 8 | 8 | 4.68 |
| P55c | 9 | 9 | 444 |
| P56c | 6 | 6 | 21.45 |
| P57c | 3 | 3 | 552 |
| P61 | 5 | 5 | 2.18 |
| P62c | 53 | 50 | 22.95 |
| Colon Cancer Family Registry | |||
| P30 | 10 | 9 | 1.49 |
| P31 | 10 | 10 | 3.67 |
| P34 | 9 | 9 | 4.4 |
| P35 | 2 | 2 | 1.87 |
| P36 | 4 | 4 | 21.89 |
| P37 | 4 | 4 | 36.41 |
a
Failed quality control due to low unique DNA start sites per gene-specific primer 2 (<50).
b
A Lynch syndrome carrier without active tumor detected at the blood collection time points.
c
Serum was used in the analysis. All the remaining cell-free DNA were from plasma.