Figure 2. Mitochondrial calcium uniporter regulates BCAA catabolism pathway.

(A) Representative immunoblots of select BCAA catabolism proteins and uniporter components and their quantification in HeLa WT and MCU KO cells; n=3. (B) Representative immunoblots of select BCAA catabolism pathway proteins and their quantification in HeLa WT and EMRE KO cells; n=3. (C) Representative immunoblots of select BCAA pathway proteins and their quantification in AML12 cells following shRNA-mediated MCU knockdown; n=3. (D) Schematic of BCAA catabolism pathway. The committed step in the pathway is catalyzed by BCKDH complex which is active in the dephosphorylated state in (A-C) was determined by one-sample t-test. All error bars represent standard deviation; * indicates a p-value < 0.05 and ** indicates a p-value < 0.01.