FIG. 3.

Expression in independent clones derived from complementation experiments. Six colonies were cloned and expanded at 32°C from either K1 and LT1-135 HQ or K1 and st cotransfections. Cell lysates were prepared and resolved by SDS-PAGE followed by Western blotting and probing with antibody PAb419, which recognizes an epitope within the first 82 amino acids of LT. Hence, st could be detected on the same blot as K1. Lanes: 1, lysate from untransfected parental T64-7B cells, included as a control; 2 to 7, lysates from K1 and st cotransfections; 8 to 13, lysates from K1 and LT1-135 HQ clones.