FIG. 4.

Cell cycle distribution in stable lines after shift to 32°C. Pools of stable lines were derived from T64-7B cells by cotransfection of various expression plasmids with a puromycin resistance vector (pCMV puro) and selection in puromycin for about 10 days. Each pool was shifted to 32°C for 24 h, and cell cycle distribution was monitored by propidium iodide staining and FACS analysis. For comparison, two of the K1+st clonal lines (2 and 5) as well as two of the K1+LT1-135 HQ clonal lines (9 and 10), grown at 32°C, were included. Percentages indicate the content of G₀/G₁, S, and G₂/M cells as estimated by using FACScan software. TAg, T antigen.