Figure 1. Modular replicative and integrative expression vectors.

(A) Circular plasmid map and features of the replicative shuttle vector containing both E. coli and A. baumannii origins of replication (top) and the Tn7 expression vector containing a transposon that will integrate into the chromosomal att_Tn7 site (bottom). Available antibiotic resistance cassettes (AbR) are listed. Maps are adapted from SnapGene (GSL Biotech). (B and C) Linear maps showing the modular promoter region and multiple cloning sites (MCS) for the replicative plasmid and Tn7 vector. NcoI site provides an ATG start codon optimally proximal to a strong ribosome binding site (RBS).