Figure 2. P_abstBR promoter construction and expression.

(A) Promoter sequences showing the homologous recombination event in lacO repeat regions (red) of the P_LlacO-1 sequence that produces P_abst, which contains a −35-like region (yellow). Site-directed mutagenesis reverts the −35 region back to consensus (orange) to create P_abstBR. (B) Dot plots showing sfGFP fluorescence from replicative vectors containing sfgfp under P_abst, P_abstBR, or P_trc promoters in A. baumannii ATCC 17978 with no IPTG (left) or 1 mM IPTG (right). Values were normalized to empty vector controls, and sample means are represented by a solid horizontal line (n=3). Asterisks and ns indicate significant and not significant sample differences, respectively (Welch’s t-tests; p-values < 0.05).