FIG. 3.

Viral protein synthesis in BHK-21 and L-929 cells. BHK-21 and L-929 cells were infected with WT GDVII (G) or dl-L (▵) at an MOI of 5 and incubated at 37°C. (A) At 8, 24, or 72 h p.i., the cells were labeled with [³⁵S]methionine for 1 h; then cell lysates were prepared and analyzed by SDS-PAGE. Lane M shows the patterns of proteins synthesized in mock-infected cells. (B) Lysates from L-929 cells radiolabeled 8 h p.i. were used for immunoprecipitation as described in Materials and Methods.