FIG. 1.

Western blot analysis of LMP1 in MEF cells after infection with v-LMP1. MEF cells were infected with either v-LNSX (MEF-Vector) or v-LMP1 (MEF-LMP1) and passaged twice before collected for analysis (P3). B95-8 is an EBV-positive marmoset B-lymphocyte cell line that expresses LMP1, and MOLT-4 is an EBV-negative lymphoblastic leukemia cell line. Cell lysates were made, and 100 μg of protein was loaded in each lane for Western blot analysis unless otherwise indicated. To compare the relative levels of LMP1 in MEF-LMP1 and B95-8 cells, two- and fourfold dilutions of both lysates were made and analyzed. A mouse monoclonal antibody against LMP1, S12, was used for the immunoblot.