FIG. 3.

In situ analysis of HCV genomic and replicative-intermediate RNAs in cell lines with Dig-labeled riboprobes. Cells in panels A and B were probed with HCV antisense riboprobes, while cells in panels C and D were probed with HCV sense riboprobes. (A) Huh7-hcvS cell lines, which express positive-strand (i.e., genomic polarity) HCV RNA. (C) Huh7-hcvAS cell lines, which express negative-strand (i.e. replicative-intermediate) HCV RNA. (B and D) Control Huh7 cell lines. Nuclear methyl green stain was used; magnifications, ×250 (A) and ×100 (B, C, and D). Arrows indicate the positive signal for HCV RNA.