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In situ analysis of HCV-infected, explanted cirrhotic liver tissue. (A, C, and E) HCV-infected liver tissue. (B, D, and F) Noninfected (normal) liver tissue. (A and B) Hybridization with antisense riboprobes. (C and D) Hybridization with sense riboprobes. (E) Hybridization with hybridization solution free of riboprobes. (F) Hybridization with human endogenous HPRT and beta-actin riboprobes. Nuclear methyl green stain was used; magnification, ×250.
