TABLE 1.
Dig-labeled riboprobes
Clone | Insert (nucleotide [nt] location) | Size of riboprobe (nt) | Primers used for RT-PCR | Reference or source |
---|---|---|---|---|
pACS | Human beta-actin cDNA (408–1064)a | 657 | AT-1 and AT-4 | 27 |
pCORE | Core gene of HCV genotype 1a (489–751)b | 263 | c104 and c186 | 30 |
pCU | HCV 5′UTR and core gene (183–513)b | 331 | Ish1 and c186 (M&M) | 30 |
pE1-1a | E1 gene of HCV genotype 1a (802–1639)b | 838 | JBE1_1S and JBE_1AS | 4 |
pE1-1b | E1 gene of HCV genotype 1b (802–1639)b | 838 | JBE1_1S and JBE_1AS | 4 |
pHR14 | Human HPRT cDNA (316–604)c | 289 | 5′HPRT and 3′HPRT | 37 |
pSPT18-neo | Bacterial neomycin transferase gene (383–1114)d | 732 | Boehringer |