Fig. 5. ESYT1 overexpression rescues bona fide inhibitory synapses on motor neurons.

a–e Inhibitory presynaptic marker Vgat and inhibitory postsynaptic marker Gephyrin co-localize in motor neurons of WT mice. e Co-localization of Vgat and Gephyrin is shown in the correlation scatterplot. f–i Both markers are downregulated in SOD1^G93A mice at P112, (j) correlation scatterplot shows dramatic decrease of Vgat and Gephyrin co-localization in the SOD1^G93A mice. k–o Co-localization of Vgat and Gephyrin in motor neurons of En1^cre mice upon ESYT1 overexpression. p–t Rescue of Vgat and Gephyrin expression in SOD1^G93A;En1^cre mice overexpressing ESYT1. t Correlation scatterplot shows increased co-localization of Vgat and Gephyrin in the SOD1^G93A;En1^cre condition. u Quantifications of the area where Vgat and Gephyrin are found co-localized in the different conditions (One-way ANOVA and Fisher’s LSD post hoc Vgat: P = 0.003; Gephyrin: P = 0.003, WT = 3, En1^cre N = 2, SOD1^G93A N = 4, SOD1^G93A;En1^cre N = 3). v Both pre- and postsynaptic inhibitory markers colocalize as indicated by Pearson’s correlation; SOD1^G93A;En1^cre mice show higher colocalization than SOD1^G93A mice and similar to WT and En1^cre controls (One-way ANOVA and Fisher’s LSD post hoc: P = 0.006, WT = 3, En1^cre N = 2, SOD1^G93A N = 4, SOD1^G93A;En1^cre N = 3). All graphs show mean values ± SEM. Source data are provided as a Source Data file.