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. 2024 Feb 28;245(1):70–83. doi: 10.1111/joa.14033

FIGURE 1.

FIGURE 1

Expression of Prkd1, Prkd2 and Prkd3 in E12.5 hearts. (a) Prkd1 mRNA expression in Prkd1 em1/+ (het) and Prkd1 em1/em1 (hom) hearts, compared to WT. A statistically significant difference in expression of Prkd1 in homozygous hearts compared to wild type (WT) (p = 0.009) and heterozygotes (p = 0.009) was found. (b) Mean normalized PRKD1 protein expression in percentage. Prkd1 em1/+ (het) and Prkd1 em1/em1 (hom) hearts were compared to WT (100%) using GAPDH as the reference protein. A significant difference was seen between homozygotes and WT controls (p = 0.017). (c) Representative western immunoblot of PRKD1 and GAPDH loading control in Prkd1 em1 E12.5 hearts. (d) Prkd2 and Prkd3 mRNA expressions in Prkd1 em1/+ (het) and Prkd1 em1/em1 (hom) hearts, compared to WT. No statistically significant difference in expression of Prkd2 or Prkd3 in homozygous hearts compared to WT (p = 0.43 and 0.52, respectively) and heterozygotes (p = 0.33 and 0.34, respectively) was found. For all experiments n = 3 per group, with each study repeated three times; error bars denote SEM; FC, fold change; n.s, not significant; **p < 0.01; *p < 0.1.