Table 4.
Culture conditions for the isolated mammary epithelial cells from mammary gland and milk
| Species | Basal media | Serum | Antibiotics | Hormones | Other additives | References |
|---|---|---|---|---|---|---|
| Buffalo | DMEM/F12 | FBS (10%) | Penicillin (100 U/mL), streptomycin (5 µg/mL), amphotericin (50 ng/mL) | INS (5 µg/mL), CORT (1 µg/mL), EGF (10 ng/mL), PRL (5 µg/mL) | Transferrin (1 µg/mL) | [45] |
| Caprine | DMEM/F12 | FBS (10%) | Penicillin (100 U/mL), streptomycin (100 µg/mL) | INS (10 µg/mL), CORT (5 µg/mL) | Sodium bicarbonate (2.2 mg/mL), sodium acetate (5 mmol/L), holo-transferrin (5 µg/mL), ethanolamine (0.5 mmol/L), | [46] |
| Dairy cow | DMEM/F12 | FBS (10%) | INS (5 µg/mL), P4 (5 µg/mL), CORT (1 µmol/L), EGF (10 ng/mL), E2 (5 µg/mL) | Transferrin (5 µg/mL) | [47] | |
| Dairy cow | DMEM/F12 | FCS (10%) | Penicillin (1%), streptomycin (1%) | INS (5 µg/mL), CORT (1 µg/mL), EGF (10 ng/mL), PRL (5 µg/mL) | Transferrin (5 µg/mL), glutamine (1%) | [48] |
| Dairy cow | DMEM/F12 | FBS (10%) | Penicillin/streptomycin (1%) | EGF (1%) | [49] | |
| Dairy cow | DMEM/F12 | FBS (20%) | Penicillin/streptomycin (50 IU/mL), amphotericin B (2.5 µg/mL) | INS (1 µg/mL) | [50] | |
| Dairy cow | DMEM/F12, INS (5 mg/mL) | FCS (10%) | Penicillin/streptomycin (100 µg/mL), gentamycin (100 µg/mL), amphotericin B (5 µg/mL) | Transferrin (5 mg/mL), sodium selenite (5 µg/mL) | [51] | |
| Dairy cow | DMEM/F12 | FBS (10%) | Amphotericin B (1.76 µg/mL) | INS (10 µg/mL), CORT (1 µg/mL), | Transferrin (5 µg/mL), sodium selenite (5 ng/mL) | [52, 53] |
| Goat | DMEM/F12 | FBS (10%) | Penicillin/streptomycin (100 IU/mL) | ITS (5 ng/mL), IGF-1 (10 ng/mL), EGF (10 ng/mL) | [54] | |
| Human | DMEM/F12 | FBS (5%) | INS (5 µg/mL), CORT (0.4 µg/mL), EGF (10 ng/mL) | Cholera toxin (8.4 ng/mL), adenine (24 µg/mL), Y-27632 (10 µmol/L) | [55] | |
| Porcine | DMEM/F12 | FBS (10%) | INS (0.5 µg/mL), CORT (1 µg/mL), PRL (0–2 µg/mL) | [56] | ||
| Yak | DMEM/F12 | FBS (10%) | Penicillin (100 IU/mL), streptomycin (5 µg/mL) | ITS (5 µg/ML), EGF (5 ng/mL), CORT (1 µg/mL), P4 (5 µg/mL) | [57] |
CORT Cortisol, DMEM/F12 Dulbecco’s Modified Eagle’s Medium/F-12 Nutrient Mixture Ham, E2 17β-estradiol, EGF Epidermal growth factor, FBS Fetal bovine serum, FCS Fetal calf serum, IGF-1 Insulin growth factor-1, INS Insulin, ITS Insulin-transferrin-selenium, P4 Progesterone, PRL Prolactin