FIGURE 2.

Tailored P(HB‐co‐HHx) production in fed‐batch mode at laboratory scale using R. eutropha Re2058/pCB113 with mixtures of fructose and canola oil as carbon sources and urea as nitrogen source. Cell dry weight (CDW, g L⁻¹), PHA content of CDW (PHA, wt%), HHx content of PHA (HHx, mol%), fructose concentration (g L⁻¹), urea concentration (urea, g L⁻¹), and fructose and canola oil feeding rates (feed, g h⁻¹) over the whole process time are shown. The ratio of fructose to canola oil (g g⁻¹) is given for each substrate mixture. The final carbon content of all cultivations was 46 g L⁻¹, and a C/N ratio of 22 g g⁻¹ was used. Error bars represent the standard deviation of biological replicates. Dashed lines indicate the feeding start (light red, dash–dot–dot) and nitrogen limitation (dark red, short dash).