TABLE 1.

Effect of anti-cathepsin D antibody on HIV-1 enhancement^a

HIV strain	Medium	Enhancement effect of:
		Buffer	Anti-cathepsin D antibody
HIV-1 IIIb	Vaginal fluid	1.6	1.1
	Cathepsin D	1.8	1
	Milk	1.4	1
SI 121	Vaginal fluid	2.6	1.4
	Cathepsin D	1.5	1
	Milk	1.6	0.8
NSI 114	Vaginal fluid	2.5	1.7
	Cathepsin D	2.1	1.5
	Milk	1.8	0.7

^aVaginal fluid diluted 1:10, milk diluted 1:10, and cathepsin D at 2 mg/ml were incubated for 10 min at 37°C with polyclonal anti-cathepsin D antibody (Biogenesis) diluted 1:500 or buffer. The mixtures were further incubated for 10 min with the three virus strains and then inoculated into lymphocyte cultures. After 10 days, levels of HIV p24 in the supernatant were measured, and enhancement was estimated by the ratio of results for treated viruses to nontreated viruses.