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. Author manuscript; available in PMC: 2024 Jun 9.
Published in final edited form as: Nat Biomed Eng. 2024 Apr 19;8(5):593–610. doi: 10.1038/s41551-024-01194-7

Extended Fig. 2. Varying the electrostatic charge of the polypeptide affects immunogenicity via ER stress-mediated mtDNA release and effector functions in macrophages.

Extended Fig. 2.

(a) Chemical structure of polypeptides with different electrolytes. P1 more favorably induced (b) ER stress and (c) mtDNA release in BMDMs greater than did PTMA (strongly cationic) and PS (anionic) (n=3, mean±SD), ordinary one-way ANOVA. P1 improved (d) phagocytosis of EO771 breast cancer cells and (e) cross-presentation of the model antigen SIINFEKL-H2Kb to a greater extent than did PTMA or PS (n=3, mean±SD), ordinary one-way ANOVA. (f) Expression of genes for pro-inflammatory cytokines was regulated by types of electrolytes and strength of cationic charges (n=3, mean±SD), ordinary one-way ANOVA.