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. Author manuscript; available in PMC: 2024 Jun 9.
Published in final edited form as: Nat Biomed Eng. 2024 Apr 19;8(5):593–610. doi: 10.1038/s41551-024-01194-7

Extended Fig. 1. Varying hydrophobicity in the engineered polypeptide affects immunogenicity via ER stress-mediated mtDNA release and effector functions in macrophages.

Extended Fig. 1.

(a) Chemical structure of cationic polypeptides with different amine-containing analogues. Cationic polypeptides including hydrophilic analogues and cyclic structures more favorably induced (b) ER stress and (c) mtDNA release in bone marrow-derived macrophages (BMDMs) [n=3, mean±standard deviation (SD)], ordinary one-way analysis of variance (ANOVA). Cationic polypeptide tethered with a hydrophilic building block and cyclic structure increased (d) phagocytosis of EO771 breast cancer cells and (e) cross-presentation of the model antigen SIINFEKL-H2Kb (n=3, mean±SD), ordinary one-way ANOVA. (f) Gene expression of pro-inflammatory cytokines was affected by hydrophobicity of polypeptides and the chemical structure of amine-including analogues (n=3, mean±SD), ordinary one-way ANOVA.