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. 2024 Jun 8;15:4913. doi: 10.1038/s41467-024-49348-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–d Statistical analysis of CD11b^hiF4/80⁺ monocyte-derived macrophages (a), neutrophils (b), monocytes (c), and CD11b^lowF4/80⁺ macrophages (d) from WT mice at different time points after P. berghei ANKA infection. n = 7-8 (a-d) Multiple comparisons test corrected for Tukey was used to test for significant differences between uninfected and infected groups. e, f Transcription of the gene coding for SOD3 was determined by RT–qPCR in neutrophils (e) and macrophages (f) after parasite infection (n = 3). An ~10-fold increase in gene transcription was observed in neutrophils but not in macrophages. Statistical significance tested by one-way ANOVA, and multiple comparisons to the uninfected control were corrected using Dunnet’s method. Three biological replicates were included in the experiment. g Representative high-magnification images of spleen sections of WT mice analyzed with double immunofluorescence for SOD3 (red) and myeloperoxidase (MPO, green). Significant augment of SOD3 expression in the splenic tissue of infected mice was observed. Arrow heads indicate representative cells with strong colocalization. Three biological replicates were included in the experiment. Scale bar =20 µm. h Comparable Western blotting analysis of SOD3 expression in neutrophils sorted from uninfected or infected mice. SOD3 expression in neutrophils of infected mice was significantly more than that from uninfected mice. Three biological replicates were included in the experiment. Each experiment was repeated three times independently with similar results. The neutrophils illustration was created with http://BioRender.com (publishing license: TB26UIITUU). i Schematic representation of the procedure for adoptive transfer. The illustration was created with http://BioRender.com (publishing license: NC26UIHFCR). j Adoptive transfer of WT neutrophils into SOD3^−/− mice resulted in early death of SOD3^−/− mice after P. berghei ANKA infection. Five biological replicates were included in the analysis. Kaplan‒Meier survival curves were calculated using the survival time for each mouse in all groups, and significance was determined by the log-rank test. Histograms present the mean ± SD. Source data are provided as a Source Data file.