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. 2024 Jun 8;15:4901. doi: 10.1038/s41467-024-49215-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The structure of Avi(Me)Cys motif and representative examples of Avi(Me)Cys-containing RiPPs, with the Avi(Me)Cys motifs highlighted in yellow. b Overview of the rule-based omics mining workflow: The central panel illustrates the common biosynthetic process for Avi(Me)Cys unit formation, which informs the genome mining of Avi(Me)Cys-containing RiPP BGCs using SPECO (left panel) and guides the metabolomics analysis (right panel) for targeted peptide identification.