Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jun 8;21:154. doi: 10.1186/s12974-024-03147-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Workflow for spinal cord-derived EV separation and characterization. a The diagram depicting the EV separation method using size exclusion chromatography and ultracentrifugation (created using BioRender.com). b Clinical scores of mice at day 16 and day 25 showed no significant difference (n = 15 per time point). c Representative electron microscopy images of negatively stained EVs from spinal cords before EAE induction and at days 16 and 25 (ten images taken for three samples from each time point). d Particle concentration and size at the three time points obtained using NTA (bar graphs show the mean ± SD of n = 3 samples (pooled from 5 spinal cords) at each time point. Statistical significance was determined using one-way ANOVA and Turkey’s post-test. ns = not significant. e Number of the top 100 EV-enriched proteins, according to Vesiclepedia and Exocarta, present in the spinal cord EV proteome. f Heatmap showing the relative abundance of commonly used EV markers in spinal cord EV samples and 10k pellet samples (EV depleted particles) obtained as a byproduct of the EV isolation. g Heatmap showing the relative abundance of frequently described possible co-isolated proteins recovered from tissue during EV separation. (n = 3 for each time point and each sample type, i.e., EV and 10k)