Figure 3.
RNA-58 cleavage by Dz or BDD cleavage agent (CA). (A) Multiple-turnover conditions: RNA-58 (1 μM) was incubated with each of the cleavage agents (0.1 μM) in Buffer 1 at 37°C for 5 h followed by the analysis of the cleavage products by PAGE. Turnover number (TON) was calculated as described in Material and Methods. (B) Single-turnover conditions: RNA-58 (1 μM) was incubated with each cleaving agent (5 μM) at 37°C for either 5- or 15-min. Single-turnover data obtained for a Dz:RNA ratio of 2:1 is presented in Supplementary Figure S2. The data is the average values of three independent experiments. The parameters TON and kobs were as described in Materials and Methods and presented for 5 h and 15 min, respectively.