Figure 4.

BDD1 and BDD3 demonstrate greater RNase H-dependent RNA cleaving than Dz1, Dz2 or Dz1 + Dz2. RNA-58 (1 μM) was incubated with each of the cleavage agents (0.1 μM) in Buffer 1 at 37 °C for 20 min with or without RNase H. The cleavage products were separated from RNA substrate by denaturing PAGE (Supplementary Figure S3) followed by quantification as detailed in Materials and Methods. (A) RNA-58 cleavage in the presence of RNase H (RNase H+). (B) RNA-58 cleavage by the Dz cleaving agents in the absence of RNase H (RNase H–). The data is the average values of three independent experiments.