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RNA cleavage activity depends on the stability of the dsDNA platform in BDDs. (A) Design of BDD constructs with different lengths of the dsDNA platform with noted number of base pairs (bp) and melting temperature (Tm). (B) RNA-58 cleavage by BDD1,3–6 under multiple-turnover conditions. TON was calculated after 5 h of incubation as described in the Materials and Methods. The data is the average values of three independent experiments.
