Figure 8.
Selectivity of RNA cleavage by different Dz agents. (A) RNA-58 was cleaved by Dz agents having one nucleotide mismatch in one (1A or 2′A) or two (12′A) arms. (B) Relative efficiency of RNA-58 cleavage under multiple-turnover conditions (5 h, 1 μM RNA, 0.1 μM Dz agents). (C) Table with selectivity factors F(s) for the RNA-cleaving BDDs and Dz with or without mismatches. F(s) was calculated as (1 – TONmm / TONm), where TONmm and TONm and the turnover numbers for mismatched and matched cleaving agents, respectively. The data is the averaged values of three independent experiments.