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. 2024 Mar 21;52(10):5529–5548. doi: 10.1093/nar/gkae183

Figure 5.

Figure 5.

H3K18la interacts with chromatin remodeling complex Brg1 in early reprogramming. (A) Pearson's correlation coefficient was analyzed to evaluate the repeatability of individual samples. (B) Principal component analysis indicated the difference between MEF and iPSC. (C) The protein was divided into four groups according to the differential expression (Q1–Q4), and Q4 represented that the protein expression in iPSC was twice that in MEF, and P< 0.05. (D)The molecular function of proteins interacted with H3K18la. (E) Protein domains interacted with H3K18la. (F) WB showed Brg1 protein level in MEF and iPSC. The histogram analysis displayed the relative statistical values of Brg1 in MEF and iPSC. Bars represent the mean and SD of n = 3 independent experiments. (G) Co-IP showed H3K18la interaction with Brg1. (H) Co-IP showed Brg1 interaction with H3K18la. (I) Interaction mode of Brg1 protein and unmodified H3 protein. (J) Interaction mode of Brg1 protein and K19 lactated modified H3 protein *P< 0.05, **P< 0.01, ***P< 0.001. t-test.