Figure 2.
Structural differences between PEDV and SARS-CoV-2 nsp12s. (A) Overlay of SARS-CoV-2 (PDB: 7KRP) and PEDV (8URB) nsp12. The black box highlights the loop region depicted in (B). (B) PEDV nsp12 loop (residues 249–268) buries extra surface area on nsp8F compared to SARS-CoV-2 (PDB: 7KRP). (C) PEDV nsp12 841–849 lacks helical definition in the absence of nsp8T, while it has a short helix when the nsp8T N-terminal extension is present. For each panel except (C) PEDV and SARS-CoV-2 models are superimposed with PEDV being colored in darker shades. In (C), nsp12 from our complex without nsp8T is colored in grey. Superimposition of models was achieved using the Matchmaker function in ChimeraX (40).