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. 2024 Mar 5;52(10):5975–5986. doi: 10.1093/nar/gkae153

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Effects of replication factor disrupting nsp12 mutations on polymerase activity. Sites of nsp12 mutations designed to disrupt replication factor interactions for (A) PEDV and (B) SARS-CoV-2 are shown as orange spheres. The effects of PEDV (C) and SARS-CoV-2 (D) nsp12 mutations were evaluated using in vitro RNA primer extension assays. Technical triplicates of reactions were run and percent activity of wildtype nsp12 is presented. Error bars indicate standard deviation of the triplicates. All reactions, except PEDV nsp12-V842R, were significantly (P< 0.05) different compared to the wildtype control. Significance was determined using an unpaired t-test for each mutant complex.