TABLE 1.
Description of AtHV-3 ORFs and comparison to SaHV-2 and HHV-8
| AtHV-3 ORF | Stranda | Position of:
|
No. of amino acids encoded | Calculated molecular mass of product (kDa) | % Identity of product with:
|
Product description/homologyb | ||
|---|---|---|---|---|---|---|---|---|
| Start/exon 1 | Exon 2/stop | SaHV-2 | KSHV (HHV-8) | |||||
| 1 | c | 90–730 | 1339–1507 | 269 | 29.2 | 32.8/40.0 | —c | Tio; homologous to StpC and Tip |
| 3 | 3224 | 6961 | 1,245 | 138.1 | 70.0 | 25.4 | FGARAT | |
| 4a | 7470 | 8552 | 360 | 40.2 | 73.1 | 28.3 | mCCPH | |
| 4b | 7470–8331 | 8525–8571 | 302 | 33.7 | 72.2 | 30.6 | sCCPH | |
| 6 | 9205 | 12591 | 1,128 | 127.4 | 86.7 | 54.9 | Major single-stranded-DNA binding protein | |
| 7 | 12598 | 14640 | 680 | 78.5 | 82.3 | 45.2 | Transport protein | |
| 8 | 14627 | 17050 | 807 | 91.1 | 85.9 | 55.7 | Glycoprotein B | |
| 9 | 17123 | 20152 | 1,009 | 114.4 | 87.5 | 60.8 | DNA polymerase | |
| 10 | 20192 | 21412 | 406 | 45.4 | 82.0 | 24.0 | Homologous to EBV Raji LF1/LF2 in B95-8 deletion, EHV-2 ORF10 | |
| 11 | 21415 | 22632 | 405 | 45.8 | 75.6 | 28.4 | Homologous to EBV Raji LF2 in B95-8 deletion, EHV-2 ORF11 | |
| 14 | c | 23207 | 24028 | 273 | 30.6 | 47.0 | — | ie14/vsag |
| 16 | 25075 | 25599 | 174 | 19.5 | 65.0 | 19.0 | bcl2 family | |
| 17 | c | 25629 | 27128 | 499 | 55.9 | 77.6 | 41.7 | Protease, minor capsid scaffold protein |
| 18 | 27121 | 27891 | 256 | 29.7 | 82.8 | 48.0 | ||
| 19 | c | 27885 | 29519 | 544 | 61.2 | 79.7 | 46.2 | Virion protein |
| 20 | c | 29149 | 30060 | 303 | 34.5 | 67.3 | 41.8 | Fusion protein |
| 21 | 30059 | 31642 | 527 | 59.7 | 76.4 | 28.1 | Thymidine kinase | |
| 22 | 31639 | 33789 | 716 | 83.2 | 87.6 | 34.3 | Glycoprotein H | |
| 23 | c | 33782 | 34549 | 255 | 29.2 | 83.7 | 33.5 | |
| 24 | c | 34559 | 36751 | 730 | 82.5 | 82.1 | 47.4 | |
| 25 | 36757 | 40872 | 1,371 | 153.8 | 89.4 | 65.0 | Major capsid protein | |
| 26 | 40888 | 41802 | 304 | 34.5 | 89.8 | 55.6 | Capsid protein VP23 | |
| 27 | 41809 | 42627 | 272 | 31.7 | 72.1 | 31.5 | ||
| 28 | 42692 | 42916 | 74 | 7.9 | 56.3 | 36.1 | ||
| 29a | c | 42929 | 43984 | 351 | 39.0 | 90.6 | 52.3 | DNA-packaging protein, terminase |
| 30 | 44089 | 44316 | 75 | 8.3 | 81.3 | 33.3 | ||
| 31 | 44283 | 44909 | 208 | 24.4 | 87.5 | 44.3 | ||
| 32 | 44855 | 46180 | 441 | 51.2 | 71.4 | 29.8 | ||
| 33 | 46173 | 47165 | 330 | 36.8 | 79.7 | 36.2 | ||
| 29b | c | 47107 | 48063 | 318 | 36.4 | 81.8 | 61.8 | |
| 29 | c | 42929–44071 | 47155–48063 | 683 | 77.0 | 87.3 | 58.9 | DNA-packaging protein, terminase |
| 34 | 48062 | 49012 | 316 | 36.2 | 87.0 | 44.3 | ||
| 35 | 48999 | 49454 | 151 | 17.4 | 76.0 | 29.9 | ||
| 36 | 49339 | 50631 | 430 | 48.4 | 81.4 | 30.2 | Phosphotransferase | |
| 37 | 50631 | 52082 | 483 | 55.9 | 87.4 | 50.9 | Alkaline exonuclease | |
| 38 | 52037 | 52237 | 66 | 7.7 | 69.7 | 40.0 | Homologous to myristylated tegument protein in EHV-2 | |
| 39 | c | 52263 | 53360 | 365 | 42.2 | 84.1 | 49.3 | Integral membrane protein |
| 40 | 53445 | 54773 | 442 | 50.7 | 68.6 | 26.9 | Helicase-primase complex | |
| 41 | 54926 | 55408 | 160 | 18.5 | 74.4 | 27.5 | Helicase-primase complex | |
| 42 | c | 55400 | 56197 | 265 | 29.7 | 80.0 | 37.7 | |
| 43 | c | 56163 | 57863 | 566 | 64.6 | 89.2 | 58.8 | Minor capsid protein, virion protein |
| 44 | 57829 | 60174 | 781 | 88.0 | 89.5 | 61.1 | Helicase-primase complex, helicase | |
| 45 | c | 60214 | 60996 | 260 | 29.1 | 61.0 | 29.9 | |
| 46 | c | 61004 | 61762 | 252 | 29.1 | 82.9 | 59.1 | Uracil DNA glycosidase |
| 47 | c | 61740 | 62162 | 140 | 15.9 | 67.2 | 32.1 | Glycoprotein L |
| 48 | c | 62159 | 64537 | 792 | 92.6 | 50.7 | 21.6 | Contains a large acidic repeat |
| 49 | c | 64768 | 65682 | 304 | 35.8 | 73.9 | 20.2 | |
| 50 | 65682 | 67250 | 522 | 58.2 | 68.7 | 21.7 | Transcriptional control; homologous to EBV Rta | |
| 51 | 67818 | 68867 | 349 | 37.0 | 40.5 | 23.9 | Virus-specific glycoprotein | |
| 52 | c | 68906 | 69253 | 115 | 13.2 | 66.7 | 24.3 | |
| 53 | c | 69295 | 69564 | 89 | 10.0 | 61.8 | 25.8 | |
| 54 | 69636 | 70499 | 287 | 32.2 | 74.9 | 35.0 | dUTPase | |
| 55 | c | 70537 | 71139 | 200 | 22.5 | 83.5 | 46.4 | |
| 56 | 71118 | 73625 | 835 | 96.0 | 81.3 | 44.3 | Helicase-primase complex, primase | |
| 57 | 73782 | 75053 | 423 | 47.5 | 76.2 | 27.6 | Transcriptional control; homologous to EBV Mta | |
| 58 | c | 75434 | 76495 | 353 | 40.7 | 83.3 | 29.3 | |
| 59 | c | 76504 | 77604 | 366 | 40.1 | 77.0 | 30.8 | Processivity factor of DNA polymerase |
| 60 | c | 77719 | 78636 | 305 | 35.3 | 92.5 | 63.0 | Ribonucleotide reductase small subunit |
| 61 | c | 78642 | 80945 | 767 | 87.0 | 90.0 | 51.9 | Ribonucleotide reductase large subunit |
| 62 | c | 80945 | 81937 | 330 | 37.5 | 86.1 | 40.1 | Probable capsid assembly, DNA maturation protein |
| 63 | 81944 | 84643 | 899 | 103.4 | 75.9 | 30.7 | Tegument protein | |
| 64 | 84643 | 92058 | 2,471 | 280.0 | 72.0 | 30.5 | Large tegument protein | |
| 65 | c | 92062 | 92463 | 133 | 14.4 | 69.7 | 29.3 | Capsid protein |
| 66 | c | 92426 | 93760 | 444 | 50.9 | 79.5 | 34.6 | |
| 67 | c | 93679 | 94380 | 233 | 26.5 | 84.1 | 50.6 | Tegument protein |
| 67A | c | 94377 | 94634 | 85 | 9.7 | 84.0 | 41.6 | |
| 68 | 94627 | 95937 | 436 | 49.3 | 77.8 | 47.0 | Probable major envelope protein | |
| 69 | 95939 | 96724 | 261 | 29.8 | 88.1 | 49.0 | ||
| 70 | c | 96897 | 97769 | 290 | 32.9 | 84.8 | 65.5 | TS |
| 71 | c | 100187 | 100528 | 113 | 13.4 | 42.5 | 28.3 | FLIP |
| 72 | c | 100529 | 101317 | 262 | 29.6 | 74.8 | 29.6 | D-type cyclin |
| 73 | c | 101349 | 102692 | 447 | 46.5 | 35.0 | 15.0 | Glycine rich, repetitive structure |
| 74 | 103148 | 104113 | 321 | 36.6 | 70.6 | 31.5 | Viral IL-8 receptor | |
| 75 | c | 104198 | 108097 | 1,299 | 143.1 | 73.6 | 36.0 | FGARAT |
a
c, complementary strand.
b
Taken in part from descriptions of other sequenced herpesvirus ORF products. Abbreviations: FGARAT, formylglycineamide ribotide amidotransferase; mCCPH and sCCPH, membrane-bound and soluble complement control protein homologs; EBV, Epstein-Barr virus; EHV-2, equine herpesvirus 2.
c
—, not present.